Fluorinated complexes doped nanodot with high brightness and dual emission for ratiometric detection of glucose-related enzymes

Abstract

Fluorescence detection of enzymes have attracted considerable interest due to their promise in biological applications, while suffering from the limitation of rigorous demand on the structure design of enzyme substrates. Herein, we report a simple and convenient approach for fluorescence detection of multiple enzymes by developing a fluorescence/phosphorescence dual-emissive nanodot, which is composed of fluorinated organic dyes and fluorinated Pt (II) porphyrin complexes, and exhibits ratiometric luminescence response toward oxygen. We characterized the optical properties of the nanodot, which showed high brightness and sensitive oxygen-response luminescence. Based on the oxygen-consumed enzymatic reaction of glucose oxidase and the glucose-generated enzymatic reaction of the enzymes related to glucose metabolism, and using this presented nanodot, the concentration of glucose and the enzymatic activity of invertase, β-galactosidase, and maltase in aqueous solution were successfully determined quantitatively. To the best of our knowledge, the strategy proposed here for enzyme detection is the first demonstration of an optical probe for monitoring the enzymatic activity of multiple enzymes. Significantly, the strategy presented here may provide a general design principle to develop a kind of luminescent probe for detecting various enzymes.