Abstract
In the present work methods for the fluorimetric determination of some quinonic and quinonoid compounds are proposed. These compounds seldom have natural fluorescence or have a very weak fluorescent signal. Primin (2-methoxy-6- n-pentyl-1,4-benzoquinone), plumbagin (2-methyl-5-hydroxy-1,4-naphthoquinone), lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-napthoquinone), tingenone and pristimerin (the last two triterpenoid quinone methides), compounds with recognized pharmacological activity, were studied from the analytical point of view after reduction with sodium dithionite or sodium borohydride in aqueous ethanolic or in ethanolic medium. Parameters such as effect of solvent, fluorophore stability, acid or base addition, excess reagent, effect of irradiation and in some cases the effect of temperature were evaluated. After optimization the calibration curves and the analytical figures of merit were determined. The limits of detection ranged from 0.16 to 210 ng ml −1.
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