Abstract
One of the goals of molecular cytogenetics applied to livestock is the extension of their genetic physical maps, especially of loci containing genes related to productions. In this study, a comparative fluorescence in situ (FISH) mapping of three genes related to fecundity of cattle, river buffalo, sheep and goat is reported using bovine BAC-clones taking in account the data available on the BovMap database and considering their physical position and the data obtained from banding experiments. The following three gene sequences were mapped: tumor necrosis factor-α (TNF), correlated to male fertility; signal transducer and activator of transcription 5A (STAT5A), important for its influence on milk production and reproduction activity; melatonin receptor 1A (MTNR1A) important for reproductive seasonality. BAC probes containing these gene sequences were assigned by FISH, for the first time, on RB-banded chromosomes of these four important bovids. TNF was assigned to BTA/CHI23q21-22, OAR20q21-22 and BBU 2p21-22; STAT5A was assigned to BTA/CHI19q17-21, OAR11q17-21 and BBU3p15-21; MTNR1A was assigned to BTA/CHI27q14-15, OAR26q14-15 and BBU1p21-22. The three loci were located in homoeologous chromosomes and chromosome bands, underling the high degree of chromosome homologies among Bovids and extending the cytogenetic maps of this economically important species.
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