Abstract
The critical roles of membranes inside cells make strategies to image and characterize them essential to fully understand cellular function. Many membrane features, including invaginations, contact sites, and microdomains, are smaller than the diffraction limit of light and as such require super-resolution imaging techniques for the detailed analysis of their dynamic structure. In this mini-review, we described lipophilic, lipid-like, and expansion microscopy (ExM)-based fluorescent molecules that have been recently developed for subdiffraction-scale imaging of membranes. By focusing on the chemical features that enable membrane targeting and super-resolution imaging, we hope to inspire future developments that will expand our view of membrane function in cells.
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