Abstract
A rapid lateral flow fluorescence microsphere immunoassay test strip (FM-ICTS) for the detection of deoxynivalenol (DON) residues in different agricultural products was established based on competitive immunoreactivity. For this purpose, two ultra-sensitive anti-DON monoclonal antibodies (mAbs) were prepared and characterized, displaying high affinity and sensitivity. mAb 3F5 had a 50%-inhibitory concentration (IC50) of 1.93 ng/mL, an affinity constant of 2.2 × 109 L/mol, and a limit of detection (LOD) of 0.29 ng/mL for DON. The mAb was covalently coupled to carboxylate modified FMs and these were used as markers in a competitive immunochromatography assay. The FM-mAbs captured on the T-line represent the fluorescence intensity detected under UV light and can be quantified by a fluorescence reader. Under optimal conditions, the visual LODs for DON by the FM immunoassay was 2.5 ng/mL and the cut-off value was 25 ng/mL and the recovery for spiked DON in agricultural samples was 90.20%-107.32%. The results show that this FM-ICTS method is simple, reliable, and sensitive, and represents a good strategy for food safety analysis and can be used for the detection of DON residues in agricultural products.
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