Abstract
AbstractThe anti myoglobin conjugated iron oxide/ru(bpy)32+/silica, anti myoglobin conjugated rhodamine 6G/silica particles were prepared, and used for myoglobin detections. The anti myoglobin conjugated iron oxide/ru(bpy)32+/silica particles were mixed with myoglobin in the micro centrifuge tube. After 10 min, the magnetic separators were introduced to catch the myoglobin‐captured anti myoglobin/iron oxide/ru(bpy)32+/silica particles from the homogenous solutions. For sandwich assays, the anti myoglobin conjugated rhodamine 6G/silica particles were incubated. The concentration of myoglobin was quantified based on anti myoglobin conjugated rhodamine 6G/silica particles. The calibration curve of the myoglobin showed the linear range to be between 1 × 10−12 and 1 × 10−10 (M) (R2 = 0.9944). The minimum detectable concentration was 17.6 pg/mL. The fluorescence method offered the best way to determine myoglobin with a total analysis time of less than 30 min.
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