Fluorescence quenching of fluorescein dye using silver nanoparticles

Abstract

Fluorescence quenching is indeed a versatile and widely used technique in various scientific disciplines, including chemistry, biology, and medicine. It provides valuable information about the behaviour of small molecules in organized systems and offers insights into the properties of the microenvironments surrounding the fluorescent probe and quencher molecules. In the present work, we report the synthesis of citrate-stabilized silver nanoparticles and also investigate the effect of the prepared silver nanoparticles on the fluorescence luminous intensity of fluorescein dye with different concentrations of silver nanoparticles. The UV–visible spectrometer is used to study the absorption properties of silver nanoparticles. We have obtained a single surface plasmon resonance band, which indicates the isotropic nature of particles. The particle size was obtained from the absorption spectra, using the Mie scattering theory. The luminous intensity of fluorescein dye was found to be quenched in addition to silver nanoparticles. The morphological analysis is done using SEM.