Fluorescence microscopy study of fluorescein in the posterior segment of the eye following local application of drugs

Abstract

After local application of gentamicin, dexamethasone and tropicamide to rat eyes, no effect on the permeability or the caliber of retinal vessels was observed, nor any diffusion of fluorescein from the choriocapillaris to the outer retinal layers. The qualitative assessment of fluorescence microscopic preparations (and photographic reproductions of them) is relatively unreliable and has led to confusion in the literature. The results presented here, which to some extent contradict earlier findings published in 1971, are discussed. The authors expect to obtain more accurate results from the quantitative method which they are now using.