Abstract
Abstract The interaction of the mutagenic dye 9-aminoacridine (9AA) with polyriboadenylic acid (poly rA) was studied by both steady-state and transient fluorescence measurements. It was found that the fluorescence decay of 9AA bound to poly d(A-T) follows a single-exponential decay law but that the decay data of 9AA bound to poly rA can be well described as a sum of two-exponential functions. The fact that nanosecond time-resolved fluorescence spectra are independent of time suggests that the double-exponential decay may be attributed to different microenvironments at the binding sites. The decay parameters, fluorescence emission maxima, and fluorescence quantum yields obtained at acid pH differ from those obtained at neutral pH. The fluorescence behavior of 9AA is discussed in connection with the conformational change of poly rA.
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