Abstract
A new improved fluorescence correlation spectroscopy method for determining motional states of particles, fluorescence correlation spectroscopy with traveling interference fringe excitation (FCSTFE), is described. With this method the modulated fluorescence signal from particles excited by an interference fringe moving at constant velocity is detected, and cosine and sine Fourier coefficients at the frequency of the traveling fringe [Fcos(t), Fsin(t)] are recorded. Autocorrelation functions of Fcos(t), Fsin(t), and the square of amplitude [A2(t)=Fcos(t)2+Fsin(t)2] were found to express the motion of the fluorescent particles. The apparatus utilizing a photon counting system was constructed, and the performance of the system was demonstrated by the determination of diffusion coefficients of dispersed fluorescent polystyrene spheres. Features of FCSTFE in particle dynamics evaluation are discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
