Abstract
A spectral imaging system was developed to study the development of breast cancer xenografts in a murine mammary window chamber model. The instrument is configured to work with either a laser to excite fluorescence or a broadband light source for diffuse reflectance imaging. Two applications were demonstrated. First, spectral imaging of fluorescence signals was demonstrated with a GFP-breast cancer tumor and fluorescein injection. Second, based on the principles of broadband reflectance spectroscopy, the instrument was used to monitor dynamic changes of tissue absorbance to yield tissue oxygenation maps at different time points during tumor progression.
Highlights
Fluorescence imaging has a wide range of applications in cancer research that include the study of metastasis and tumor invasion [1], investigation of interaction of cancer cells with the microenvironment of the host [2,3], cellular receptor detection [4], and glycolytic metabolism [5,6]
The development of an orthotopic mouse mammary window chamber (MWC) model [9,10,11] has facilitated the use of optical techniques to investigate the dynamic spatiotemporal changes of breast cancer xenografts in vivo
Fluorescence spectral imaging in the MWC was demonstrated with green fluorescent protein (GFP)-breast cancer xenografts and subsequent fluorescein injection
Summary
Fluorescence imaging has a wide range of applications in cancer research that include the study of metastasis and tumor invasion [1], investigation of interaction of cancer cells with the microenvironment of the host [2,3], cellular receptor detection [4], and glycolytic metabolism [5,6]. Optical spectroscopy of tissue endogenous chromophores in tissue, most commonly blood constituents, allow the investigation of oxygenation status and its effect on treatment response [7,8]. The development of an orthotopic mouse mammary window chamber (MWC) model [9,10,11] has facilitated the use of optical techniques to investigate the dynamic spatiotemporal changes of breast cancer xenografts in vivo. The MWC model enables serial multi-modality imaging of a tumor as it develops from its initial stages and is a powerful platform with which to study many aspects of tumor biology and response to treatment. An optical spectral imaging system is developed to study breast cancer development in a murine MWC model. Fluorescence spectral imaging in the MWC was demonstrated with GFP-breast cancer xenografts and subsequent fluorescein injection. The instrument was used to monitor dynamic changes of tissue absorbance, which were subsequently used to yield tissue oxygenation maps at different time points during tumor progression
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