Abstract
ABSTRACTAn ammonia limited chemostat culture ofGymnodinium simplex (Lohm.) Kofoid & Swezy was perturbed with ammonia and fluctuations in the free intracellular amino acid pools were followed 80 min. The steady‐state value of glutamate was 2.07 ± 10‐15mol cell‐1and of glutamine was 0.31 ± 10‐15mol cell‐1. Five minutes after the perturbation, a substantial rise in glutamine was observed with a corresponding decrease in glutamate. This is considered a result of glutamine synthetase acting as the primary ammonia assimilating enzyme. The level of ammonia and the major free amino acids reached a maximum 10 min after the perturbation and then slowly decreased.
Published Version
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