Abstract
Fluorimetric determination of the DNA content of nuclei in apices of latent buds of the grapevines (cv. Shiraz) revealed an increase in the proportion of 2C-nuclei during Anlage formation and differentiation. There was a predominance of 2C-nuclei in apices and Anlagen in plants grown at high temperature (30 °C day — 25 °C night). Nuclei with the 4C-level of DNA occurred with greater frequency in apices and Anlagen formed at low temperature (18 °C day — 13 °C night). Fully formed inflorescence — and tendril primordia contained more 4C-nuclei than 2C-nuclei. Histochemical tests in cryostat sections of latent bud apices and Anlagen indicated greater activity of acid phosphatase and peroxidase in the peripheral zone cells than in the rib meristem. Intense activity of acid phosphatase was found in cells of apices which are involved in the formation of organs (leaf primordia, Anlagen and inflorescence primordia). The activity of acid phosphatase was less in Anlagen from plants grown at low temperature (18 °C day — 13 °C night) than in plants grown at high temperature (30 °C day — 25 °C night). Accumulation of starch was found mostly in the diaphragm cells and in older leaf primordia of latent buds.
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