Abstract
On stem segments of Begonia franconis LIEBM. in vitro, only vegetative buds could be induced. On inflorescence pedicels, on the contrary, the formation of adventitious flower buds was abundant. Both cytokinin and auxin were required, the latter during the first 10 days. After the promotion of callus formation, the auxin became inhibitory to flower bud formation. Gibberellin strongly repressed flower bud development. None of the growth regulators tested affected the ratio of male to female flower buds. Pistillate buds never differentiated directly on the callus, but originated at the bracts on the pedicels of the staminate ones. A high sucrose level was required for female differentiation, particularly. It is concluded that the sex of Begonia franconis LIEBM. flower buds is determined by the nutritional state of the tissue as a limiting factor for female differentiation.
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