Abstract
A flow-injection system for the chemiluminometric determination of d-glucose in serum with an immobilized pyranose oxidase reactor is described. Pyranose oxidase is immobilized on controled-pore glass beads and packed into a stainless-steel column. Serum (100 nl) is diluted 1000-fold with water. Sample solution (10 μl) is injected into the carrier stream. The hydrogen peroxide is detected by measuring the chemiluminescence emitted on admixing with luminol and potassium hexacyanoferrate(III). The maximum sample throughput is 60 h −1. The calibration graph is linear from 0.2 to 500 μM glucose; the detection limit is 0.05 μM. The immobilized enzyme reactor is stable for at least 2 months.
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