Abstract
A new flow injection chemiluminescence (CL) method is proposed for the determination of thiamine, based upon its enhancing effect on the CL reaction of luminol with hydrogen peroxide in alkaline solution. The method allows the determination of thiamine within 0.05–8 μg ml −1 range with a detection limit (3 σ) of 0.01 μg ml −1. The relative standard deviation is 1.4% ( n=11, 0.5 μg ml −1 thiamine) and the sample throughput is about 90 samples h −1. The method was successfully applied to the determination of thiamine in pharmaceutical preparations.
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