Abstract

The outbreaks of the Zaire ebolavirus (ZE) disease (ZED) that have arisen in the last decade determine the need to study the infection pathogenesis, the formation of specific immunity forming as well as the development of effective preventive and therapeutic means. All stages of fight against the ZED spread require the experimental infection in sensitive laboratory animals, which are rhesus monkeys in case of this disease .The aim of the study is to evaluate the rhesus monkey cellular immunity following the ZE experimental infection by the means of flow cytometry (cytofluorimetry). Male rhesus monkeys were intramuscularly infected by the dose of 15 LD50 (dose of the pathogen that causes 50% mortality of infected animals) of the ZE, the Zaire strain (ZEBOV). Levels of 18 peripheral blood lymphocyte populations of the animals before the ZE experimental infection and at the terminal stage of the disease were assessed using flow cytometry. The certain changes in the levels of the lymphocyte populations were observed following infection, indicating simultaneous activation and suppression of the immune system during ZED. The increase in content was observed for T-lymphocytes, T-helper and cytotoxic T-lymphocytes expressing the corresponding markers of early activation. The decrease was recorded for T-lymphocytes and double-positive T-lymphocytes expressing corresponding markers of late activation, as well as natural killer cells expressing CD8 (p < 0.05). For the first time in the Russian Federation, the rhesus monkey cellular immunity before and after the ZE experimental infection was assessed using flow cytometry.

Highlights

  • The outbreaks of the Zaire ebolavirus (ZE) disease (ZED) that have arisen in the last decade determine the need to study the infection pathogenesis, the formation of specific immunity forming as well as the development of effective preventive and therapeutic means

  • All stages of fight against the ZED spread require the experimental infection in sensitive laboratory animals, which are rhesus monkeys in case of this disease

  • Male rhesus monkeys were intramuscularly infected by the dose of 15 LD50 of the ZE, the Zaire strain (ZEBOV)

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Summary

НАУЧНАЯ СТАТЬЯ

Оценка клеточного иммунитета макаков резусов методом проточной цитометрии после экспериментального инфицирования вирусом Эбола (Filoviridae; Ebolavirus: Zaire ebolavirus). Борисевич Г.В.1, Кириллова С.Л.1, Шатохина И.В.1, Лебедев В.Н.1, Шагарова Н.В.1, Сыромятникова С.И.1, Андрус А.Ф.1, Ковальчук Е.А.1, Кириллов В.Б.1, Беспалов М.Л.2, Петров А.А.1, Ковальчук А.В.1, Пантюхов В.Б.1, Кутаев Д.А.1, Борисевич С.В.1, Кузнецов С.Л.3. Цель исследования – оценка клеточного иммунитета макаков резусов методом проточной цитометрии (цитофлуориметрии) (ПЦ) после экспериментального инфицирования ВЭ. Для цитирования: Борисевич Г.В., Кириллова С.Л., Шатохина И.В., Лебедев В.Н., Шагарова Н.В., Сыромятникова С.И., Андрус А.Ф., Ковальчук Е.А., Кириллов В.Б., Беспалов М.Л., Петров А.А., Ковальчук А.В., Пантюхов В.Б., Кутаев Д.А., Борисевич С.В., Кузнецов С.Л. – концепция и дизайн исследования, сбор и обработка материалов, написание текста; Кириллова С.Л.

ORIGINAL RESEARCH
ORIGINAL ARTICLE
Introduction
Материал и методы
Уайта White
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