Flow cytometric dna content analysis of paraffin-embedded tissue derived from cervical carcinoma

Abstract

Purpose: Flow cytometric deoxyribonucleic acid (DNA) content analysis has been shown to be of prognostic importance in some cancers. There have been recent reports of a prognostic importance for DNA content analysis in cervical carcinoma. Methods and Materials: We retrospectively reviewed the hospital and radiation oncology records of cervical carcinoma patients who presented between 1984–1990. Results: A total of 101 archival paraffin-embedded blocks were processed, of which 77 were of technical quality for analysis. Thirty-five percent were found to be DNA content aneuploid (DNA-A) and 65% DNA content diploid (DNA-D). No statistical difference was found between the two groups in age at diagnosis, % S-phase, coefficient of variation (CV), or proliferative index (PI). A statistical difference was noted in the G 2M phase between the two groups ( p = 0.004). The median % S-phase was 8.4% in the DNA-D group. A statistical difference ( p = 0.017) in survival was noted between the low and high % S-phase DNA-D groups. In patients who received radiation alone, high-PI patients had improved survival compared to low-PI patients. No statistical difference in survival was noted in the high % S-phase DNA-D group and DNA-A group ( p = 0.28). Proportional Hazard (Cox) Regression found clinical stage the only independent prognostic indicator for survival. Conclusion: Flow cytometric DNA content analysis is being used more frequently in the management of different malignant tumors. Our study shows that DNA content analysis is useful in determining the prognosis and survival outcomes in cervical carcinomas and may aid in predicting outcome to certain types of treatment regimens.