Flow-Batch Method with a Sequential Injection System for Spectrophotometric Determination of Selenium(IV) in Selenium-Enriched Yeast Using o-Phenylenediamine

Abstract

A precise, accurate, and reliable flow-batch spectrophotometric method for the determination of selenium (IV) was developed using o-phenylenediamine as a reagent with a sequential injection monosegmented flow system incorporating a simple heating unit. The reaction zones of selenium(IV) and o-phenylenediamine were mixed and heated in a chamber at 62°C for 5 minutes. The piaselanol complexes were then detected at a maximum absorption wavelength of 335 nm. In-line single standard calibration and standard addition procedures were developed employing the monosegmented flow technique. Under the optimized conditions, a linear calibration graph in a range of 0.1–4.0 mg L−1 selenium (IV) was obtained with limits of detection and quantitation of 0.01 and 0.1 mg L−1, respectively. Relative standard deviations were 2% [for both 0.1 and 0.5 mg L−1 selenium (IV) (n = 11)]. A sample throughput of 2 h−1 using four standard addition levels was achieved. The developed system was successfully applied to raw selenium-enriched yeast samples. The analyses performed by the developed method agreed well with those obtained from a standard inductively coupled plasma mass spectrometry method.