Floods enhance the abundance and diversity of anthropogenic microparticles (including microplastics and treated cellulose) transported through karst systems

Abstract

Microplastics (plastics <5 mm) are emerging contaminants that have been detected in virtually all environments. While microplastic research in terrestrial surface waters has been proliferating, microplastic contamination in subsurface environments remains understudied. Karst terrains may be particularly susceptible to microplastic pollution because the presence of large dissolution openings allows fast transport of water through these systems, facilitating the introduction of surface contaminants into subsurface habitats. Furthermore, few studies address the prevalence and movement of microparticles composed of semisynthetic and modified natural materials, despite their known ecotoxicity. Our study therefore aims to identify anthropogenic (i.e., synthetic, semisynthetic, and treated natural) microparticle extent, sourcing, and transport in subsurface karst environments. To do so, we examined a cave spring under variable flow conditions, finding that anthropogenic microparticles were present in all samples and were most frequently fibrous and clear. The mean anthropogenic microparticle concentration during baseflow was 9.2 counts/L but increased up to 81.3 counts/L during floods, indicating their enhanced mobilization when relatively dilute, acidic, and sediment-rich event water entered the cave. These results suggest that anthropogenic microparticles may originate from surface recharge or sediment resuspension within the cave. When we analyzed a subset of microparticles with Fourier transform infrared spectroscopy (FTIR), we found that cellulose of known (i.e., dyed) and suspected (i.e., clear) anthropogenic origin was the most abundant material type. We nevertheless confirmed the presence of microplastics in the cave stream under all flow conditions, with the most common polymer being polyethylene. Both the concentrations and relative fractions of microplastics were higher during floods compared to baseflow, indicating their increased transport during high flow events. We also observed that microplastic polymer types diversified as discharge increased. Our study gives new insight into how anthropogenic microparticle contamination is transported through karst landscapes that can help inform debris mitigation strategies to protect ecosystems and water resources.