FLAVONOIDS AND PHENILPROPANOIDS OF THE ABOVE GROUND ORGANS OF THE BUPLEURUM MUL-TINERVE DC. OF THE FLORA OF THE BAIKAL REGION

Abstract

A study of the flavonoids and phenylpropanoids of the aerial organs of Bupleurum multinerve was carried out. Samples of raw materials were collected in the Baikal region in the vicinity of. Verkholensk, Irkutsk region. The raw materials were collected during the flowering period in 2019 in the herb-grass association. For analysis, raw materials were extracted with 70% ethyl alcohol in an ultrasonic bath for 30 minutes, followed by centrifugation of the extract. Compounds were identified by the UPLC-DAD-ESI-MS method in comparison with standard samples. In the aerial organs of B. multinerve, 15 compounds were identified (8 flavonoids and 7 phenylpropanoids). Flavonoids B. multinerve are derivatives of quercetin, kempferol, isoramnetin; phenylpropanoids – esters of quinic acid with coffee, ferulic, coumaric. The contents of B. multinerve compounds were first established: quercetin-3-O-glucuronide, kempferol-3-O-glucoside (astragaline), 5-O-p-coumaroylquinic acid, 5-O-feruloylquinic acid, 3-O-feruloylquinic acid, 3,5-di-O-caffeoylquinic acid; 4,5-di-O-caffeoylquinic acid. The quantitative content of glycosides of flavonoids isoramnetin-3-O-rutinoside (narcissin) 20.74 mg/g, quercetin-3-O-rutinoside (rutin) 19.53 mg/g, quercetin-3 was determined by MK-HPLC-UV in the aerial organs of B. multinerve -O-glucoside (isocvercitrin) 2.24 mg/g, camperol-3-O-glucoside (astragaline) 0.39 mg/g, quercetin-3-O-glucuronide 0.25 mg/g; quercetin flavonoid aglycones 0.43 mg/g; isoramnetin 0.53 mg/g; the predominant phenylpropanoids were 5-O-caffeoylquinic acid 6.60 mg/g, 3,5-di-O-caffeylquinic acid 1.58 mg/g. The total content of identified flavonoids was 44.97 mg/g, phenylpropanoids 9.53 mg/g.