Abstract
Summary Glucose esters of hydroxycinnamic acids often occur in association with quinic and other acid esters. In a number of surveys these two forms of cinnamic acid derivatives could not be satisfactorily distinguished from each other. The present paper describes the separation of sugar esters and quinic acid esters by means of polyamide column chromatography. Clones of Spirodela polyrrhiza were cultured axenically on a defined medium in a controlled environment room. Lyophilized fronds were thoroughly extracted with methanol. The filtered extract was diluted with water to 80% methanol, shaken against tetrachloromethane and the epiphase evaporated to aqueous consistence. The extract was fractionated on polyamide SC, yielding a water, methanol and methanolic NH 3 eluant. The methanol fraction only contained flavone derivatives. 1-feruloylglucose and 1-sinapoylglucose were found in the water fraction, while 5-p-coumaroylquinic acid and 5-caffeoylquinic acid were detected in the NH 3 eluant. All these compounds were found in Spirodela polyrrhiza for the first time. Identifications were confirmed by spectrophotometry, chromatography and co-chromatography with authentic compunds.
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