Flavonoid-Rich Extract of Paeonia lactiflora Petals Alleviate d-Galactose-Induced Oxidative Stress and Restore Gut Microbiota in ICR Mice.

Lei Liu,Jun Tao,Yingdan Yuan

doi:10.3390/antiox10121889

Lei Liu, Jun Tao + Show 1 more

Open Access

https://doi.org/10.3390/antiox10121889

Copy DOI

Abstract

This study was aimed to investigate the antioxidant effect of Paeonia lactiflora Pall. petal flavonoids extract (PPF) on d-galactose (d-gal)-induced ICR mice. In this study, sixty male ICR mice were randomly divided into six groups during an 8 weeks experimental period, including normal control (NC) group, d-gal group, epigallocatechin gallate (EGCG) group, low, medium, and high dose PPF groups (10, 20 and 40 mg/kg/day). The results showed that intragastric administration with PPF significantly reverses the atrophy of the visceral organs of oxidative damage mice in a dose-dependent relationship. PPF indicated the antioxidant capacity to decrease the malondialdehyde (MDA) level and improve the activity of superoxide dismutase (SOD), catalase (CAT) as well as glutathione peroxidase (GSH-Px). In addition, PPF treatment reversed gut microbiota dysbiosis by increasing the relative abundance of Lactobacillaceae. Spearman correlation analysis showed that the body’s oxidative stress markers were directly related to changes in gut microbiota. These findings reveal firstly that PPF could alleviate d-Gal-induced oxidative stress and modulate gut microbiota balance.

Highlights

The potential harm of oxidative stress to human beings and animals has attracted extensive attention
The present study evaluated the antioxidant capacity of Pall. petal flavonoids extract (PPF) in vivo, investigated the underlying mechanism, and explored the correlation between the gut microbiota and antioxidant status in the D-gal-induced oxidative stress mouse model
The study revealed that Paeonia lactiflora petal flavonoids can alleviate oxidative stress by increasing the activity of superoxide dismutase (SOD), CAT, and GSH-Px and decreasing the level of MDA in mice

Summary

Introduction

The potential harm of oxidative stress to human beings and animals has attracted extensive attention. Oxidative stress is a gradual process from the time of birth [1], and the balance of the oxidation and antioxidants is broken with the gradual increase in reactive oxygen species (ROS) in the organism. Maintaining the normal oxidation-reduction balance of the organism is essential to the overall health of human beings. According to the reported antioxidant mechanism, living organisms remove excessive oxygen free radicals by scavenging ROS precursors, chelating with metal ions involved in the formation of ROS, and increasing endogenous antioxidant defenses in the body [4]. Given the important impact of oxidative stress on living organisms, adding various antioxidant supplements into the daily diet is essential to antagonize oxidative stress and maintain normal body organs

Objectives

Methods

Results

Discussion

Conclusion