Flat panel airlift photobioreactors for cultivation of vegetative cells of microalga Haematococcus pluvialis

Abstract

The novel flat panel airlift photobioreactor (FP-ALPBR) was proposed as an alternative system for the cultivation of Haematococcus pluvialis NIES-144. Changes in the efficiency of the system were tested in response to variations in two engineering parameters: the ratio between the downcomer and riser cross-sectional areas ( A d / A r ) and the size of the system (as determined by the length of the panel) and to one operating parameter: the superficial gas velocity ( u sg ). The best growth performance was obtained by operating the system at a superficial velocity of 0.4 cm s −1 and with a downcomer-to-riser cross-sectional area ratio of 0.4. The 17-l FP-ALPBR system was capable of giving reasonable growth characteristics with a maximum cell density of 4.1 × 10 5 cell ml −1 and specific growth rate of 0.52 day −1 being achieved. A similar level of performance was obtained from the 90-l FP-ALPBR system, i.e., cell density = 40 × 10 4 cell ml −1 but with a slight decrease in specific growth rate to 0.39 day −1. The performances of these two differently sized FP-ALPBRs were compared with two conventional cylindrical airlift photobioreactors (C-ALPBRs) of different dimensions. The 90-l FP-ALPBR exhibited reasonably good performance when compared with the two 17-l systems (both C- and FP-ALPBRs); however, the best growth rate was observed using the 3-l C-ALPBR. Semi-continuous cultures, which could be periodically harvested at a reasonably high growth rate, were successfully created. Of all the systems investigated in this study, the 90-l FP-ALPBR was found to be the most cost-effective, as it could cultivate 18 g of alga for approximately US$ 21.