Fixings Agent Effect to β-carotene Stabilityof Crude Extract from Dunaliella salina Microalga at Different Temperature Condition

Abstract

Abstract<br />Dunaliella salina is a microalga rich pigment carotenoids that can be used asnatural dyes. The problem<br />faced for temporative β-carotene stability which is unstable and easily degraded is unstable and easily<br />degraded. The purpose ofthis researchwas to determine content and stability of β-carotene pigment in<br />D.salina with fixings addition of MgCO3 and NaHCO3 with temperature treatment as factor that influence<br />β-carotene pigment degradation during storage condition. The research method used β-carotene pigment<br />which was obtainedbymaceration and adding fixing agent (NaHCO3 0,5% and without fixings addition),<br />and was heated at temperature of (50°, 70° and 90°C), be measured the content of β-carotene pigment<br />during storage (observation hour 0, 24, 48, 72, 96, 120 and 144). Data analyzed by ANOVA and Honestly<br />Significant Different (HSD). The result showed that content of β-carotene pigment at 50°C temperature<br />showed that stability and pH value performed slowest degradation after144 hours. While stability and pH<br />value at 90°C temperature performed fastest degradation after 96 hours. Based on this study the addition of<br />NaHCO3 gave more effective in retaining β-carotene degradation compared to that of MgCO3 which showed<br />by high stability in sample NaHCO3 0,5%, temperature 50°C in 144 hours storage length.<br /><br />