Abstract
The development of biomaterials with the potential to accelerate wound healing is a great challenge in biomedicine. In this study, four types of samples including pepsin soluble collagen sponge (PCS), acid soluble collagen sponge (ACS), bovine collagen electrospun I (BCE I) and bovine collagen electrospun II (BCE II) were used as wound dressing materials. We showed that the PCS, ACS, BCE I and BCE II treated rats increased the percentage of wound contraction, reduced the inflammatory infiltration, and accelerated the epithelization and healing. PCS, ACS, BCE I, and BCE II significantly enhanced the total protein and hydroxyproline level in rats. ACS could induce more fibroblasts proliferation and differentiation than PCS, however, both PCS and ACS had a lower effect than BCE I and BCE II. PCS, ACS, BCE I, and BCE II could regulate deposition of collagen, which led to excellent alignment in the wound healing process. There were similar effects on inducing the level of cytokines including EGF, FGF, and vascular endothelial marker CD31 among these four groups. Accordingly, this study disclosed that collagens (PCS and ACS) from tilapia skin and bovine collagen electrospun (BCE I and BCE II) have significant bioactivity and could accelerate wound healing rapidly and effectively in rat model.
Highlights
Skin trauma especially severe wound is a common clinical problem, and is more challenging to cure
On day 3, each group showed different degree of collagen absorption and wound areas of Bovine collagen sponge (BCS), pepsin soluble collagen sponge (PCS), acid soluble collagen sponge (ACS), bovine collagen electrospun I (BCE I) and bovine collagen electrospun II (BCE II) treated groups were obviously smaller than control and woundplast groups; on day 7, the wounds of the groups treated with BCS, PCS, ACS, Bovine collagen electrospun (BCE) I, and BCE II contracted further and scabbed, the wounds of control and woundplast groups had only a small amount of granulation tissue; on day 14, dried blood fall-off from the wound and the wounds healed completely
The wounds which were treated with PCS, ACS, BCE I, and BCE II were smooth and no pigmentation
Summary
Skin trauma especially severe wound is a common clinical problem, and is more challenging to cure. The foremost aim for the treatment of skin defects is to rapidly restore the construction and function of the wound to the levels of normal tissue, involving acute and chronic inflammations, cell division, migration, and differentiation, regeneration and vascularization [1]. Wound healing is a multifactorial process that is characterized by angiogenesis, collagen deposition, granulation tissue formation and re-epithelialization. All these phases involve complex biomolecular interactions among cells, soluble cytokines, adherence factors and chemokines. The clinical treatment of skin wound by traditional medicine has a long history from ancient times, the major drawback
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
