Abstract
In 2022, during a survey of plant-parasitic nematodes affecting hemp in Washington and Oregon, a Pratylenchus sp. was found parasitizing hemp roots. Morphology and morphometrics, as well as molecular techniques, confirmed the identity of the nematodes extracted from hemp roots as Pratylenchus penetrans. A pathogenicity assay done in the greenhouse confirmed hemp as a host to P. penetrans, with an average reproduction factor of 2.2 at 60 days postinoculation. To our knowledge, this is the first report of P. penetrans on hemp in the United States.
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