Abstract
A high number of second stage juveniles of the root-knot nematode were recovered from soil samples collected from a corn field, located in Pickens County, South Carolina, USA in 2019. Extracted nematodes were examined morphologically and molecularly for species identification which indicated that the specimens of root knot juveniles were Meloidogyne hispanica. The morphological examination and morphometric details from second-stage juveniles were consistent with the original description and redescriptions of this species. The ITS rRNA, D2-D3 expansion segments of 28S rRNA, intergenic COII-16S region, nad5 and COI gene sequences were obtained from the South Carolina population of M. hispanica. Phylogenetic analysis of the intergenic COII-16S region of mtDNA gene sequence alignment using statistical parsimony showed that the South Carolina population clustered with Meloidogyne hispanica from Portugal and Australia. To our best knowledge, this finding represents the first report of Meloidogyne hispanica in the USA and North America.
Highlights
There have been no verified reports of this nematode in North America
Meloidogyne hispanica belongs to the Ethiopica group of root-knot nematodes, which contains the species M. ethiopica, M. luci and M. inornata (Alvarez-Ortega et al, 2019)
The objective of this work was to provide mor phological and molecular characterization of this rootknot nematode isolated from corn in South Carolina, identified as Meloidogyne hispanica
Summary
There have been no verified reports of this nematode in North America. Meloidogyne hispanica belongs to the Ethiopica group of root-knot nematodes, which contains the species M. ethiopica, M. luci and M. inornata (Alvarez-Ortega et al, 2019). The objective of this work was to provide mor phological and molecular characterization of this rootknot nematode isolated from corn in South Carolina, identified as Meloidogyne hispanica. This report represents the first record of this species in the USA and North America
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