Abstract
ABSTRACT The genus Hedychium consists of about 50 species, with increasing popularity as ornamentals and potential as medicinal crop plants, but there are no reports on somatic embryogenic regeneration of any member of this genus. The objective of this investigation was to establish an in vitro regeneration system based on somatic embryogenesis for Hedychium muluense R.M. Smith using shoot apex-derived callus. Callus was induced and proliferated on a modified Murashige and Skoog (MS) medium (CIPM) supplemented with 9.05 μM 2–4, D, and 4.6 μM kinetin. Friable callus developed into somatic embryos upon transfer to liquid medium (MS basal salts and Gamborg's vitamins) that was supplemented with 0.6 μM thidiazuron (TDZ) and 8.9 μM 6-benzylaminopurine (BA) and shaken for four weeks. The cultures were then transferred to three Hedychium embryo-development media (HEDM) of varying strengths: HEDM, 1/2 HEDM, and 1/4 HEDM. All three media contained both 0.6 μM TDZ and 8.9 μM BA. Somatic embryo production was higher in full strength HEDM, which produced an average of 103 somatic embryos/explant, half of which could be converted into shoots within a month. Regenerated shoots were readily rooted on a medium supplemented with 0.6 μM 3-indoleacetic acid (IAA) and acclimatized before transfer to the greenhouse.
Published Version
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