First report of Pelargonium zonate spot virus from wild Brassicaceae plants in Japan

Abstract

Pelargonium zonate spot virus (PZSV) was identified from two wild Brassicaceae plant species, Arabidopsis halleri and Rorippa indica, in central Japan using RNA-Seq and RT-PCR. The deduced amino acid sequences of RNA-dependent RNA polymerase and coat protein were highly similar to those of previously reported PZSV isolates, with 96.6–98.2% and 93.7–98.0% identity, respectively. Mechanical inoculation revealed the pathogenicity of the PZSV isolate to Nicotiana benthamiana and Brassica oleracea. To the best of our knowledge, this is the first report of PZSV from Japan.