Abstract
European mountain ash ringspot-associated virus (EMARaV) is widespread in Germany, Finland, northwestern Russia (Karelia), and has also been reported from Austria. Disease symptoms are light rings, spots or variegation of the host leaves. In 2010, mountain ash trees (Sorbus aucuparia) showing ringspot symptoms were found in Prague. Total RNAs were extracted from 100 mg leaf tissues of 25 trees using the silica-capture method (Rott and Jelkmann, 2001). RT-PCR tests were performed using primers that amplify fragments of all four viral RNAs (Mielke et al., 2008). Products of predicted length were obtained from all symptomatic but not from symptomless plant samples. The partial EMARaV putative nucleoprotein gene fragments of two Czech isolates were amplified (Kallinen et al., 2008) and sequenced in both orientations (accession No. FR751461 and FR751462). Comparisons of nucleotide and deduced amino acid sequences of the isolates with sequences from GenBank No. GU563319, GU563318, EU885293, EU885292 and NC_013108, showed identities ranging from 97 to 99% at the nucleotide level and 100% at the amino acid level. In the course of a small scale survey conducted in central Bohemia, infected trees were found in 6 of the 12 localities checked, usually with a rather high incidence (up to 70% of tree populations), suggesting that EMARaV may be rather widespread in this country. To our knowledge, this is the first evidence of EMARaV occurrence in the Czech Republic.
Published Version
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