First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.

T P Nicholson,R J Cox,T J Simpson,C Winfield,J Crosby,J Westcott

doi:10.1039/b300847a

First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.

T P Nicholson, R J Cox + Show 4 more

https://doi.org/10.1039/b300847a

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Journal: Chemical communications (Cambridge, England)	Publication Date: Feb 19, 2003
Citations: 39

Affiliation: University of Bristol

#LCMS Spectra #Polyketide Synthase #Point Mutations #Biosynthesis Of Compounds #Actinorhodin Polyketide Synthase #Starter Units #Malonyl CoA #Supplementary Information #Experimental Procedures #Chain Length

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The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

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First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.