Abstract
Pseudomonas aeruginosa strains L25 and M12 were isolated from hospital effluent in southern Brazil. The whole genomes of the isolates were sequenced using an Illumina MiSeq system. The data were analysed using SPAdes, Prokka and Geneious, and antimicrobial resistance genes were predicted using ResFinder. PubMLST protocols were used to define the sequence type (ST). Many multidrug efflux pump systems as well as various antimicrobial resistance genes were identified in the two P. aeruginosa strains. The strains were identified as ST2963, a novel carbapenem-resistant sequence type. Here we describe the genome sequences of two carbapenem-resistant P. aeruginosa strains and characterised a novel sequence type (ST2963).
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