Abstract
Human coronavirus HKU1 (HCoV-HKU1) was first detected in a patient with viral pneumonia from Hong Kong in 2004. Here, we report the first complete genome sequence of HCoV-HKU1 from Thailand, obtained from a nonill person who worked in a bat cave. Phylogenetic tree analysis revealed it as a group B HCoV-HKU1.
Highlights
There are four species of endemic human coronavirus (HCoV) currently recognized by the International Committee for the Taxonomy of Viruses, namely, HCoV-OC43, -229E, -NL63, and -HKU1, and two epidemic CoVs, including severe acute respiratory syndrome (SARS)-CoV and the Middle East respiratory syndrome (MERS)-CoV discovered in 2003 and 2012, respectively (1)
The quality and quantity of the DNA library was estimated by using the QIAxcel Advanced system and QIASeq library quantification kit, respectively (Qiagen, Germany)
A total of 6,475,827 reads mapped to HCoV-HKU1 at a depth of 29,000ϫ, which covered the whole genome of 29,811 bp
Summary
There are four species of endemic human coronavirus (HCoV) currently recognized by the International Committee for the Taxonomy of Viruses, namely, HCoV-OC43, -229E, -NL63, and -HKU1, and two epidemic CoVs, including severe acute respiratory syndrome (SARS)-CoV and the Middle East respiratory syndrome (MERS)-CoV discovered in 2003 and 2012, respectively (1). RNA was extracted using a QIAamp viral RNA mini kit (Qiagen, Germany), followed by DNase treatment. A DNA library was prepared using a TruSeq total RNA with Ribo-zero globin kit (Illumina). The quality and quantity of the DNA library was estimated by using the QIAxcel Advanced system and QIASeq library quantification kit, respectively (Qiagen, Germany).
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