Fine structure of the gills of some Indian air-breathing fishes.

Abstract

A detailed account is given of the structure of the gills of Clarias batrachus, Heteropneustes (= Saccobranchus) fossilis, Channa punctata, Monopterus (= Amphipnous) cuchia and Boleophthalmus boddaerti, based upon light and electron microscopy. In all five species the basic organization into primary and secondary lamellae is apparent but the latter are very much more modified in Monopterus. Three main layers separate the water and blood on the surface of the secondary lamellae. The outer epithelium is usually two layered but may be multilayered close to the origin of the secondary lamellae from the gill filament. The basement membrane is relatively thin and a middle dense layer containing collagen fibrils separates two clear layers. The pillar cells, so characteristic of secondary lamellae, are present in all except Monopterus and flanges from these cells surround the blood channels with the exception of the marginal channels. The latter are lined by endothelial cells which line all the blood channels of Monopterus. The overall thickness of the three layers comprising the water/blood barrier ranges from 1.5 to 13 microns. A number of modifications to this basic organization can be related to the degree of dependence of the different species on air-breathing. Boleophthalmus is the only species commonly found in brackish water and its secondary lamellae have well developed lymphoid spaces between two layers of the epithelium. Special densely-stained regions of the pillar cell flanges were also present in this fish and may have a supporting function.