Ontogenic Development Of Gills In Pre To Post-Flexion Stages Larvae Of Himalayan Snow Trout Schizothorax Plagiostomus (Heckel)

Abstract

The ontogenic development of gills, including gill arch, gill filaments, and gill rakers in pre to postflexion stages larvae of Himalayan Snow Trout Schizothorax plagiostomus were studied with the objectives that this study could serve as a base for further studies about the early embryonic development and organogenesis in various fish species inhabiting hill stream environments. To obtain the pre to post-flexion stages larvae, an artificial breeding experiment was conducted during September- October on the bank of snow-fed river Alaknanda by stripping method. Further, the development of the gill apparatus was studied histologically, using light microscopy. Hatchling takes place 124-130 hours after fertilization at the incubation temperature of 19-200C. On second dph (day post-hatching), gill arches, gill filaments, and branchiostegal membrane began to differentiate. By the third dph, blood channels were observed in gill filaments as well as in pseudobranch. Formation of secondary lamellae, branchial arteries, elongation of the gill cover, cartilaginous rod formation in the gill arch, and the branchiostegal membrane was discernible by fourth-fifth dph. Pillar cells and afferent and efferent arteries with RBCs in primary and secondary lamellae were observed on the seventh dph. Around the onset of exclusive exogenous feeding (twelfth dph), gill rakers and a group of pillar cells with blood channels were recognizable. Well-organized and functional gill structures with increased number and size of secondary gill lamellae were present among the fifteenth-seventeenth dph larvae. The general pattern of structural and functional development of both the natural site and laboratory-reared larvae was similar, except a large amount of mucous and clustered epithelial cells among the laboratory-reared larvae, which may be due to the physiological as well as environmental stress posed by the adverse physicochemical conditions.