Abstract
BackgroundHigh yield and quality determine the commercial potential of rice variety. Brown rice rate (BRR) is a key factor ensuring grain yield and quality in rice. So far, there were few reports about the genes that directly controlled the BRR in rice.Therefore, dissecting the genetic mechanism of the BRR genes can facilitate improving effective rice supply or edible grain yield.ResultsA double haploid population derived from the cross between Taichung Native 1 (TN1) (an indica variety) and Chunjiang 06 (CJ06) (a japonica variety) was used to investigate the genetic basis of grain milling and appearance traits affecting the BRR. By using a constructed molecular linkage map, four quantitative trait loci (QTLs) for the BRR were detected on chromosomes 1, 8, 9, and 10, respectively. In addition, three QTLs for appearance traits, including grain weight and grain length/width ratio, were detected on chromosomes 6, 9 and 10, respectively. Chromosome segment substitution lines (CSSLs) were established at the qBRR-10 locus. Finally, the qBRR-10 was narrowed to a 39.5 kb region on chromosome 10. In this region, two candidate genes, LOC_Os10g32124 and LOC_Os10g32190, showed significantly differential expression in TN1 and CSSL1-2 compared with CJ06. Histocytological analysis suggested that cell size and hull thickness may be important factors for the BRR.ConclusionIn the study, the qBRR-10 affected the BRR and was finally located to a region between two markers, P13 and P14. Two candidate genes were selected based on the expression difference between two parents, which facilitated the further cloning of the qBRR-10 gene and largely contributed to improve the grain yield and quality in rice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0076-7) contains supplementary material, which is available to authorized users.
Highlights
High yield and quality determine the commercial potential of rice variety
Two candidate genes in this region showed significant expression differences in Taichung Native 1 (TN1) and CSSL1-2 compared with Chunjiang 06 (CJ06)
LOC_Os10g32124 and LOC_Os10g32190 showed significantly different expression levels in TN1 and CSSL1-2 compared with CJ06 (Fig. 6 and Additional file 1: Table S3)
Summary
High yield and quality determine the commercial potential of rice variety. Brown rice rate (BRR) is a key factor ensuring grain yield and quality in rice. There were few reports about the genes that directly controlled the BRR in rice. Dissecting the genetic mechanism of the BRR genes can facilitate improving effective rice supply or edible grain yield. Rice is a major food staple for human. It is urgent to secure grain production, which can be achieved by increasing grain yield and quality (Rosegrant and Cline, 2003). The weight of brown rice is directly related to yield. Rice hulls play a vital role in grain yield, they cannot serve as food for human. BRR is an important trait in rice, which is
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