Abstract
Key message A gene not only control tiller and plant height, but also regulate panicle structure by QTL dissection in rice. An ideal panicle structure is important for improvement of plant architecture and rice yield. In this study, using recombinant inbred lines (RILs) of PA64s and 93-11, we identified a quantitative trait locus (QTL), designated qPPB3 for primary panicle branch number. With a BC3F2 population derived from a backcross between a resequenced RIL carrying PA64s allele and 93-11, qPPB3 was fine mapped to a 34.6-kb genomic region. Gene prediction analysis identified four putative genes, among which Os03g0203200, a previously reported gene for plant height and tiller number, Dwarf 88 (D88)/Dwarf 14 (D14), had three nucleotide substitutions in 93-11 compared with PA64s. The T to G substitution resulted in one amino acid change from valine in 93-11 to glycine in PA64s. Real-time PCR analysis showed expression level of D88 was higher in 93-11 than PA64s. The expression of APO1 and IPA1 increased, while GN1a and DST decreased in 93-11 compared with PA64s. Therefore, D88/D14 is not only a key regulator for branching, but also affects panicle structure.Electronic supplementary materialThe online version of this article (doi:10.1007/s00299-014-1661-0) contains supplementary material, which is available to authorized users.
Highlights
Plant architecture is very important for improving rice yield, grain quality, resistance to multiple biotic and abiotic stresses, and nutrient utilization efficiency
With a BC3F2 population derived from a backcross between a resequenced recombinant inbred lines (RILs) carrying PA64s allele and 93-11, qPPB3 was fine mapped to a 34.6-kb genomic region
quantitative trait locus (QTL) analysis of panicle branches was performed with a RIL population derived from an indica/ javonica cross, and a major QTL for primary panicle branch number was detected on chromosome 3
Summary
Plant architecture is very important for improving rice yield, grain quality, resistance to multiple biotic and abiotic stresses, and nutrient utilization efficiency. APO1, a pivotal gene to regulate primary panicle branch, controlled the vascular bundle formation and could increase the harvest index and grain yield in rice (Terao et al 2010). QTL analysis of panicle branches was performed with a RIL population derived from an indica/ javonica cross, and a major QTL (qPPB3) for primary panicle branch number was detected on chromosome 3. The region of qPPB3 was narrowed by map-based cloning strategy with BC3F2 population derived from a chromosome segment substitution line (CSSL). Multiqtl.com) using maximum likelihood interval mapping (MIM) based on a permutation test (1,000 permutation, P = 0.05) for each dataset to confirm the LOD threshold It was considered as a major effect QTL when its LOD was larger than 2.5. We acquired a BC3F2 population for fine mapping of qPPB3
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
