Abstract
Introduction: Low postnatal serum insulin-like growth factor 1 (IGF1) and IGFBP3 are associated with severe ROP and may help predict ROP risk. For assays, blood-spot cards may offer advantages over venous blood draws. We sought to develop filter-paper bloodspot IGF1 and IGFBP3 assays and establish validity and reliability across varying storage times and temperatures for ranges expected in the ROP at-risk population.
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