Abstract
<b>Abstract ID 25450</b> <b>Poster Board 282</b> The unique physiochemical properties and tunable surface reactivity of gold nanoparticles made them promising materials in bionanomedicine. Although they are not themselves serious toxicants, however, they produce either direct or indirect nucleic acid (DNA and RNA) damages. Yet, the actual mechanism(s) are not fully understood. Investigating their real time damaging mechanism(s) on a single nucleic acid molecule (DNA in this study), in native environment, is essential to understand their genotoxicity. Hence, we applied the four dimensions Atomic Force Microscopy, as an imaging and nano-characterization tool, to explore their actual pathway(s) of DNA damaging, in vitro, in the absence of Particle-Induced Reactive Oxygen Species (ROS). To further examine whether the size and chemical conjugation of these AuNPs are properties that control their toxicity, we exposed the individual DNA molecules to small (∼10 nm diameter) naked AuNPs, large (∼22 nm diameter) naked AuNPs and large conjugated (∼39 nm diameter) AuNPs. Our results provided real time molecular dynamic evidences that all AuNPs can cause fast (in minutes), direct, time-dependent DNA damages in vitro, in the absence of ROS and may help in designing efficient nanomedicines for viral treatment, cancer therapy and bio-diagnosis.
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