Field trials evaluating the efficacy of porcine epidemic diarrhea vaccine, RNA (Harrisvaccine) in the Philippines.

Abstract

Porcine epidemic diarrhea (PED) is a devastating enteric disease causing economic losses in many countries including the Philippines. To control PED, apart from oral administration of minced intestinal materials, there are still no effective control methods. The ability of porcine epidemic diarrhea vaccine RNA particle platform (PED-RP) to induce antibody in colostrum and milk samples was investigated in two pig herds with a differing PED status in the Philippines. Herd-A was naïve but herd-B was endemically infected with PED. Sera, colostrum, and milk samples were collected prior to and following vaccination, and assayed for the presence of antibody by viral neutralization (VN) and IgG and IgA levels by ELISA spike protein. The results from both herds, compared to the non-vaccinated control group, demonstrated significantly increased VN titers and IgG and IgA levels in colostrum and milk samples of sows at 0, 7, 14, and 21days post parturition. Additionally, piglets from vaccinated sows had VN titers, and IgG and IgA levels are significantly higher than those from non-vaccinated sows. In conclusion, the results of the study demonstrate that PED-RP can be used to induce a satisfactory antibody response in colostrum and milk, as measured by VN titers and IgG and IgA levels.