Abstract
In Europe, cowpox virus (CPXV) infection in South American camelids occurs as a so-called spill-over infection. Although infected animals generally have a mild form of the disease and survive, cases of fatal generalised CPXV infection have also been described. Prevention by prophylactic vaccination is the only way to protect animals from disease. In the present study, modified vaccinia virus Ankara (MVA) vaccine, which has been successfully used in many animal species, was used in a prime-boost vaccination regimen in two alpaca herds with a history of CPXV infection. The focus of the study was the prevention of further clinical cases, and to determine the safety and immunogenicity of the MVA vaccine in alpacas. The MVA vaccine was well tolerated and safe in the 94 animals vaccinated. An indirect immunofluorescence assay (IFA) using MVA as an antigen showed that the seroprevalence of antibody after booster vaccination was 81.3% in herd I and 91.7% in herd II. Detectable antibody titres declined to 15.6% in herd I and 45.8% in herd II over a 12-month period after booster vaccination. Animals could be divided into four groups based on individual antibody titres determined over one year: Group 1 consisted of 19.3% of animals that were seropositive until the end of the trial period; Group 2 consisted of 58.0% of animals that were seropositive after booster vaccination, but seronegative one year later; Group 3 consisted of 14.7% of animals that were not seropositive at any time point; and Group 4 consisted of 7.9% of animals that were seropositive after initial immunisation, seronegative six months later, but seropositive or intermediate in IFA one year after immunisation, likely because of natural exposure. In new-born crias born to MVA-vaccinated mares, specific maternal antibodies were detected in 50.0% of animals up to 14 weeks of age. Our results confirm that MVA vaccination is a feasible tool for the prevention of CPXV disease in alpacas. Long-term studies are needed to verify future vaccination regimen in CPXV affected herds.
Highlights
Cowpox is a disease caused by the cowpox virus (CPXV), a member of the genus Orthopoxvirus (OPV), family Poxviridae [1]
Animals could be divided into four groups based on individual antibody titres determined over one year: Group 1 consisted of 19.3% of animals that were seropositive until the end of the trial period; Group 2 consisted of 58.0% of animals that were seropositive after booster vaccination, but seronegative one year later; Group 3 consisted of 14.7% of animals that were not seropositive at any time point; and Group 4 consisted of 7.9% of animals that were seropositive after initial immunisation, seronegative six months later, but seropositive or intermediate in immunofluorescence assay (IFA) one year after immunisation, likely because of natural exposure
The present study describes the application of the modified vaccinia virus Ankara (MVA) vaccine in a prime-boost immunisation regimen in two alpaca herds in Germany with a history of CPXV infections [16]
Summary
Cowpox is a disease caused by the cowpox virus (CPXV), a member of the genus Orthopoxvirus (OPV), family Poxviridae [1]. Being endemic in Eurasia, a number of animal species have been shown to be naturally susceptible to CPXV [1]. Certain non-native animal species, kept in zoos or other animal holdings, are susceptible to CPXV infections. Infection of SACs with CPXV leads to two different disease presentations: mild, mostly self-limiting infections with localised skin lesions (pustules and crusts), and generalised, frequently lethal infections with multifocal to diffuse skin lesions (papules, pustules, crusts, ulcers) accompanied by virus replication in other organs. Llamas and alpacas are an established species of livestock and pets in Europe, there are currently no data on the incidence of CPXV infection in SACs
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
