Abstract
Objective Observe the effect of EBV-LMP2 specific cellular immune response after immunization with recombinant modified vaccinia virus ankara (MVA) vaccine MVA-Lac-LMP2.Methods Immunize BALB/c mice with recombinant MVA vaccine carrying EBV-LMP2 gene (MVA-Lac-LMP2) in three groups,which were low dosage (2 × 105pfu/mouse),medium dosage (2 × 106 pfu/mouse) and high dosage(2 × 107pfu/mouse).The mice were immunized at weeks 0 and 2.One week after the final immunization,analyze the LMP2 specific CTL level of the mouse spleen lymphocytes with IFN-γ ELISPOT essay.Results Immunization of BALB/c mice with MVA-Lac-LMP2 could induce LMP2 specific CTL response.Which medium dosage and high dosage tends to elicit more potent immune response.Conclusion MVA-Lac-LMP2 could induce LMP2 specific CTL response in mice.The potency of immune responses is related with the dosage of immunization. Key words: Herpesvirus 4, human; Cenes, LMP2; Carrier proteins; Vaccinia virus; Immunity, cellular
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