Abstract
Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.
Highlights
Wound healing is a dynamic process that consists of hemostasis, inflammation, proliferation and remodeling [1]
The extracellular matrix (ECM) fibronectin outside the cell that is linked to the actin cytoskeleton inside the cell via the focal adhesions (FAs) is closely associated during the process of wound healing with the dynamic control of cell adhesion and thence directed cell migration
The integrin signals of U2OS cells was not suppressed when cells were plated on homo or porcine fibronectin treated with PNGase F, compared to being plated on untreated fibronectin (Figure 5B). These findings indicate that the adhesion enhancement effect of N-linked glycans with respect to plasma fibronectin involves a mechanism that revolves around various cell surface molecules, such as glycoconjugates or lectins, and involves cooperative integrin-fibronectin engagement; this leads to the modulation of cell adhesion
Summary
Wound healing is a dynamic process that consists of hemostasis, inflammation, proliferation and remodeling [1]. Cell protrusions that are characterized by actin polymerization being to form a dense actin network; these are extended in the direction of migration, which is followed by attachment of the www.impactjournals.com/oncotarget protrusions to the ECM fibronectin. This in turn forms nascent adhesions (new-born FAs). The ECM fibronectin outside the cell that is linked to the actin cytoskeleton inside the cell via the FAs is closely associated during the process of wound healing with the dynamic control of cell adhesion and thence directed cell migration
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