Fibroblast-like cells derived from ips cells of patients with the familial forms of parkinson’s disease can serve an effective feeder for derivation and cultivation of new ips cells lines

Abstract

Induced pluripotent stem cells iPSC can serve a highly informative model of hereditary and sporadic forms of human diseases In prospect such cells might be used in substitutive and regenerative therapy of a wide spectrum of diseases for treatment of traumas and thermal injuries At present mouse embryonic fibroblasts are frequently used for obtaining and culturing human ES and iPS cells whereas a real risk of transmission of xenogenic infectious pathogens makes it impossible to use these cells in regenerative medicine It was shown that iPSC obtained from patients with familial forms mutations in PARK and PARK genes of Parkinson s disease can effectively differentiate into fibroblast like cells derivatives similar to iPSC generated from healthy donors It was demonstrated that the obtained derivatives can be effectively used as feeder layers not only to maintain the pluripotency of autologous and allogeneic iPSC but also to obtain new patient specific iPSC lines