Abstract
ABSTRACTWe have synthesized highly exfoliated graphene oxide (GO) and used it for the fabrication of electrochemical immunosensor. The thin films of GO were spin coated onto ITO/glass substrate and thereafter Bovine Serum Albumin (BSA) antibodies (Ab) were immobilized using EDC-NHS binding chemistry to design immunobioelectrode. The graphene oxide films were characterized using different spectroscopic techniques viz FT-IR, UV-Vis spectroscopy and HRTEM. The electrochemical activity of designed immunoelectrodes was studied by cyclic voltammetry (CV) that revealed a quasi reversible behaviour of graphene oxide films. The differential pulse voltammetry (DPV) measurements of the fabricated immunoelectrodes showed successful detection of analyte at very low concentration ranges (50 fg/mL–100 ng/mL). The present study entails the potential of GO for the development of various immunosensors for different diseases.
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