Fetal life malnutrition was not reflected in the relative abundances of adiponectin and leptin mRNAs in adipose tissue in male mink kits at 9.5weeks of age.

Connie F Matthiesen,Anne-Helene Tauson

doi:10.1186/s13028-016-0250-3

Connie F Matthiesen, Anne-Helene Tauson

Open Access

https://doi.org/10.1186/s13028-016-0250-3

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Abstract

BackgroundMalnutrition in fetal life and during suckling have in some animal studies resulted in adaptive changes related to the fat and glucose metabolism, which in the long term might predispose the offspring for metabolic disorders such as obesity later in life. The objective was to study the effect of fetal life malnutrition in male mink on the gene expression of leptin and adiponectin in different adipose tissue sites.ResultsThirty-two male mink, strict carnivore species, exposed to low (FL) or adequate (FA) protein provision the last 16.3 ± 1.8 days of fetal life and randomly assigned to a low (LP) or adequate (AP) protein diet from 7 to 9.5 weeks of age were used. Adipose tissues (subcutaneous, perirenal and mesenteric) were analyzed using qPCR. Fetal life or post-weaning protein provision did not affect the relative abundances of leptin and adiponectin mRNAs in adipose tissue at 9.5 weeks of age. Relative abundances of leptin and adiponectin mRNAs were different between adipose tissue sites and were significantly higher in subcutaneous than in perirenal and mesenteric tissues.ConclusionFetal life protein malnutrition in male mink, did not result in adaptive changes in the gene expression of leptin and adiponectin mRNAs in adipose tissue at 9.5 weeks of age as found in rodents. However, both leptin and adiponectin mRNAs were significantly differently expressed between tissue sites.

Highlights

Malnutrition in fetal life and during suckling have in some animal studies resulted in adaptive changes related to the fat and glucose metabolism, which in the long term might predispose the offspring for metabolic dis‐ orders such as obesity later in life
Present study investigated if the relative abundances of leptin and adiponectin mRNAs, both playing a key role in lipid and energy metabolism, were affected post-weaning by fetal life malnourishment
The relative abundance of leptin mRNA was not affected by FL protein provision which corresponded with the plasma leptin concentration and chemical body composition [4]

Summary

Introduction

Malnutrition in fetal life and during suckling have in some animal studies resulted in adaptive changes related to the fat and glucose metabolism, which in the long term might predispose the offspring for metabolic dis‐ orders such as obesity later in life. A strict carnivore, to low protein provision in utero has resulted in lower birth weights [1, 2] and changes in the gene expression of fructose-1,6-biphosphatase, pyruvate kinase, *Correspondence: aht@sund.ku.dk 1 Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegardsvej 3, 1870 Present study investigated if the relative abundances of leptin and adiponectin mRNAs, both playing a key role in lipid and energy metabolism, were affected post-weaning by fetal life malnourishment.

Results

Conclusion