Fetal dermis inspired parallel PCL fibers layered PCL/COL/HA scaffold for dermal regeneration

Abstract

Fetal-type dermal wound healing is amazingly quick and immaculate, mainly representing the absence of scarring as well as regeneration of dermal appendages. Here, the study reported a fetal dermis inspired architectural scaffold designing for scarless dermal regeneration. The scaffold composed of a two-compartment architecture with polycaprolactone/collagen/hyaluronic acid (PCL/COL/HA) porous scaffold down and oriented parallel PCL fibers membrane top was fabricated successively through porogen-leaching and electrospinning methods. Scanning electron microscopy (SEM) showed the scaffold had hierarchical structures, including an interconnected 3D porous structure down and an oriented parallel fibers network top. The PCL/COL/HA composite scaffold showed a porosity of 76.5% and a contact angle of 72.9 degree, demonstrating desirable hydrophilic properties and wettability. In vitro and in vivo data demonstrated the two-compartment architectural scaffold facilitated fibroblasts proliferation and showed no excessive inflammatory reaction, denoting good biocompatibility and a prospect of tissue engineering in vivo. The fetal dermis inspired architectural scaffold demonstrated here can be a desirable template for dermal regeneration.