Fertility and Reproductive Pathways in Diploid and Triploid Miscanthus sinensis

Abstract

Miscanthus sinensis Andersson is a popular ornamental grass and has additional potential as a bioenergy crop. In some regards, the ability of M. sinensis to withstand a broad range of climatic and cultural conditions is desirable, but its propensity to rapidly colonize open and disturbed environments has allowed it to naturalize and become weedy in some regions in the United States. Considering the value of this crop, the development and documentation of infertile clones would be desirable. Triploid plants were evaluated for male and female fertility using pollen viability staining and seed set and germination, respectively. Pollen viability staining, seed set, and seed germination from triploid plants were reduced compared with diploids but varied considerably within each cytotype. Overall, relative female fertility of individual triploids clones [(% seed set × % germination for triploid)/(% seed set × % germination for diploid control)] was reduced substantially and ranged from 49% to 0.7%. Additionally, the reproductive pathways of triploid plants were examined by evaluating the 2C genome sizes of progeny derived from open pollination. The limited progeny arising from open-pollinated triploids were predominantly aneuploids with 2C genome sizes intermediate between diploids and triploids. There was no clear evidence of apomixis, selfing, or triploid × triploid fertilization events observed among triploid parents. Formation of unreduced gametes was rare for both ploidy levels (≈1%). The considerable reduction in female fertility in some triploid clones combined with the limited production of primarily aneuploid progeny provides highly infertile alternatives to existing diploid cultivars.