Ferric oxide nanosheet-engineered Mg alloy for synergetic osteosarcoma photothermal/chemodynamic therapy

Abstract

• Fe 3 O 4 nanosheets were prepared on Mg alloy by transforming Mg-Fe LDH. • Fe 3 O 4 nanosheets are composed of dense nanoparticles. • Fe 3 O 4 nanosheets enhanced corrosion resistance and biocompatibility of Mg alloy. • Fe 3 O 4 nanosheet-engineered Mg alloy showed PTT/CDT synergetic antitumor property. Osteosarcoma (OS) is a malignant tumor with a high rate of recurrence. Recently, biodegradable Mg-based implants have become a new therapeutic platform for bone-related diseases. However, poor biosafety and deficient intelligent tumor-killing ability of Mg-based implants are still the main challenges for the precise treatment of OS. Herein, based on the excellent catalytic and photothermal conversion properties of nanozyme ferric oxide (Fe 3 O 4 ), a novel two-step hydrothermal method for in situ preparation of Fe 3 O 4 nanosheets on the surface of plasma electrolytic oxidation (PEO)-treated Mg alloy using Mg-Fe layered double hydroxides (Mg-Fe LDH) as precursor was proposed. Compared with Mg alloy, there were no obvious corrosion cracks on the surface of Fe 3 O 4 nanosheets-coated Mg alloy (Fe 3 O 4 -NS) immersed in 0.9 wt.% NaCl for 14 days, which demonstrated the corrosion resistance of Mg alloy was significantly enhanced. Cytocompatibility experiments and hemolysis assay confirmed the great biocompatibility of Fe 3 O 4 -NS, especially, hemolysis ratio was lower than 1%. Meanwhile, Fe 3 O 4 -NS presented excellent catalytic oxidation capacity in the presence of H 2 O 2 , and its temperature can significantly increase from 27 °C to approximately 56 °C under NIR irradiation. Therefore, intelligent responsive Fe 3 O 4 nanosheets-engineered Mg-based implants demonstrated excellent antitumor properties in vivo and in vitro due to their photothermal and chemodynamic synergetic effects. This study provides a novel approach for the preparation of Fe 3 O 4 coatings on the surface of Mg alloys and a new strategy for the treatment of OS.